Flow Cytometry analysis of Hela cells using anti-PPP1R12A antibody (PB9737).
Overlay histogram showing Hela cells stained with PB9737 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PB9737,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U251 cells using anti-PPP1R12A antibody (PB9737).
Overlay histogram showing U251 cells stained with PB9737 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PB9737,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of SiHa cells using anti-PPP1R12A antibody (PB9737).
Overlay histogram showing SiHa cells stained with PB9737 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PB9737,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).
PPP1R12A was detected in immunocytochemical section of U251 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.